The Yahoo! User Interface Library (YUI) is an open-source JavaScript library for building richly interactive web applications using techniques such as Ajax, DHTML and DOM scripting. YUI includes several core CSS resources. It is available under a BSD License. Development on YUI began in 2005 and Yahoo! properties such as My Yahoo! and the Yahoo! front page began using YUI in the summer of that year. YUI was released for public use in February 2006. It is actively developed by a core team of Yahoo! engineers.

In September 2009, Yahoo! released YUI 3, a new version of YUI rebuilt from the ground up to modernize the library and incorporate lessons learned from YUI 2. Among the enhancements are a CSS selector driven engine for retrieving DOM elements, a greater emphasis on granularity of modules, a smaller seed file that loads other modules when necessary, and a variety of syntactic changes intended to make writing code faster and easier.

The YUI Library project at Yahoo! was founded by Thomas Sha and sponsored internally by Yahoo! co-founder Jerry Yang; its principal architects have been Sha, Adam Moore, and Matt Sweeney. The library's developers maintain the YUIBlog; the YUI community discusses the library and implementations in its community forum.

Also associated with the YUI Library, the YUI Theater, founded and managed by Eric Miraglia, provides worldwide access to more than 50 talks by notable speakers on JavaScript and web development.